发明名称 |
USE OF ALKALINE WASHES DURING CHROMATOGRAPHY TO REMOVE IMPURITIES |
摘要 |
In certain embodiments, the invention provides a method of purifying a protein of interest from a mixture which comprises the protein of interest and one or more contaminants, said method comprising: a) subjecting the mixture to a first chromatography matrix, wherein the protein of interest binds to the first chromatography matrix; b) contacting the first chromatography matrix with a first wash solution which has a pH of at least 9.0, and does not comprise arginine or an arginine derivative; and c) eluting the protein of interest from the first chromatography matrix into an elution solution. |
申请公布号 |
WO2016149088(A1) |
申请公布日期 |
2016.09.22 |
申请号 |
WO2016US21984 |
申请日期 |
2016.03.11 |
申请人 |
BRISTOL-MYERS SQUIBB COMPANY |
发明人 |
WANG, Jue;JAFFE, Neil E.;PATEL, Krina |
分类号 |
B01D15/20;B01D15/38;C07K1/22;C07K16/18 |
主分类号 |
B01D15/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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