| 摘要 |
- 39 ILLUMINATION SYSTEMS AND DEVICES FOR FOURIER PTYCHOGRAPHIC IMAGING A system for forming an image (110) of a substantially translucent specimen (102) has an illuminator (108) configured to variably illuminate the specimen from a plurality of angles of illumination such that (a) when each angle (495) at a given point on the specimen is mapped to a point (445) on a plane (420) perpendicular to an optical axis (490), said points on the plane have an increasing density (e.g. 1IC, 1IE, 12C, 12E, 13A, 14A, 14C, 14E, 15A, 15C, 15E) towards an axial position on the plane; or (b) the illumination angles are arranged with a substantially regular pattern in a polar coordinate system (Fig. 13A,13B) defined by a radial coordinate that depends on the magnitude of the distance from an optical axis and an angular coordinate corresponding to the orientation of the angle relative to the optical axis. A detector is configured to acquire a plurality of variably illuminated, relatively lower-resolution intensity images (104) of the specimen based on light emitted from the illuminator according to variable illumination and filtered by an optical element (109). A processor is arranged to computationally reconstruct a relatively higher-resolution image of the specimen by iteratively updating overlapping regions (1005) of the relatively higher-resolution image in Fourier space (Fig. lOB) with the variably-illuminated, lower-resolution intensity images. 452614 1 P126F64 qnpri Inrgprd 500 Start 500----- Load specimen in 510 microscope 520 Position specimen For each selected 540 illumination configuration Set selected illumination configuration Capture lower resolution image & store No '570 Done? +YesN 580 Generate higher resolution image from captured images Output Stop Fig. 5 |
