| 摘要 |
PROBLEM TO BE SOLVED: To improve an accuracy of peptide identification based on a mass spectrum obtained by MALDI-ISD analysis without performing MSanalysis.SOLUTION: A mass spectroscopy method includes: analyzing a sample obtained by adding a reference material to peptide as analysis object and preparing the mixture thereof using a matrix, and a reference sample obtained by only preparing the same reference material using the matrix, by MALDI-ISD analysis respectively; performing mass calibration to each ISD mass spectrum using ion peak positions of the reference material and the matrix and known mass of the materials (S1-S6); and acquiring a difference mass spectrum by subtracting the ISD mass spectrum of the reference sample from the ISD mass spectrum of the sample (S7). Since ion peaks derived from the reference material and the matrix are removed, the fragment ion peak of an analysis object compound can be easily observed. The mass spectroscopy method further includes identifying the peptide by serving the peak information for database retrieval(S8-S9), thereby resulting in improved identification accuracy. |
