Method of Attenuating Porcine Pseudorabies Virus, Attenuated Strains of Porcine Pseudorabies Virus, Vaccine Composition and Use Thereof

摘要

This invention provides a method of attenuating porcine pseudorabies virus, which can effectively and reproducibly attenuate porcine pseudorabies virus. The attenuated strain of porcine pseudorabies virus by use of said method can provide effective immunization for pigs.

主权项

1. A method of attenuating porcine pseudorabies virus, comprising:
(1) a step of cultivating the pseudorabies virus adapted to cell culture, wherein the pseudorabies virus is inoculated into subcultured mammalian cells, and then subcultured for at least five passages so as to obtain the porcine pseudorabies virus strain adapted to the subcultured mammalian cells; and (2) a step of attenuating the porcine pseudorabies virus, the porcine pseudorabies virus strain adapted to the subcultured mammalian cells are inoculated into subcultured avian cells and then subcultured for at least one passage so as to obtain the attenuated strain of the porcine pseudorabies virus.