QUANTITATIVE LIGANDOMICS FOR SYSTEMATIC IDENTIFICATION OF THERAPEUTIC LIGANDS

摘要

The present invention is directed to methods for systematic identification of cellular ligands, disease-associated ligands, age-related ligands and receptor-specific ligands. Disease-associated ligands are promising targets to develop novel ligand-based therapies. The methods are broadly applicable to any type of cells or diseases in in vitro and in vivo settings. This invention further used the methods to identify Scg3 as a disease-related angiogenic factor for the therapy of diabetic retinopathy, diabetic macular edema, proliferative diabetic retinopathy, vascular age-related macular degeneration, diabetic foot and cancers.

主权项

1. A high throughput ligand screening method, comprising the steps of:
(a) phage display to enrich cellular ligands, including cell-binding ligands or phagocytosis ligands; (b) next generation DNA sequencing (NGS) to identify all enriched ligands; (c) binding or phagocytosis activity quantification of all identified ligands (d) quantitative activity comparison of entire ligand profiles.