SELECTION MARKER-FREE RHIZOBIACEAE-MEDIATED METHOD FOR PRODUCING A TRANSGENIC PLANT OF THE TRITICUM GENUS

摘要

The present invention provides an improved method for producing a transgenic plant of the Triticum genus comprising the steps (a) Rhizobiaceae-mediated transformation of at least one cell of a plant of the Triticum genus with a genetic component and (b) regeneration of a transgenic plant of the Triticum genus from a transformed cell, wherein from step (a) to step (b) there is no selection of a transformed cell based on a property mediated by the genetic component or a portion thereof.

主权项

1. A method of producing a transgenic plant of the Triticum genus, comprising the steps of:
(a) transforming at least one cell of a plant of the Triticum genus with a genetic component by co-culturing cells of an explant of said plant with at least one bacterial cell from the Rhizobiaceae family, wherein said bacterial cell comprises the genetic component, and (b) regenerating a transgenic plant of the Triticum genus from at least one transformed cell or from at least one transformed cell derived from at least one transformed cell produced in step (a), wherein in step (a) and step (b), there is no selection of the at least one transformed cell based on a property imparted by the genetic component or a portion thereof