| 摘要 |
FIELD: medicine.SUBSTANCE: invention relates to a method for selection of cultivation component batches to be used in cultivation of a mammalian cell expressing a protein of interest, wherein at least two different components are employed in cultivation comprising following steps: a) providing spectra of different batches of a first component obtained with a first spectroscopic method and spectra of a second component obtained with a second different spectroscopic method and cultivation supernatant yield of protein of interest obtained in a cultivation using combinations of said different batches of first and the second component; b) identifying a relation of fused spectra of said two different spectroscopic methods after computing spectra PCA scores with the yield of cultivation; c) providing a spectrum of a further batch of first component obtained with first spectroscopic method and a spectrum of a further batch of second component obtained with second spectroscopic method; d) selecting combination of first component and second component if predicted cultivation supernatant yield based on relation of fused spectra after computing spectra PCA scores identified in b) is within +/-10 % of mean yield provided in a).EFFECT: using declared method enables to select batch of components subjected to use when culturing mammal cells expressing protein.6 cl, 11 tbl, 21 dwg, 1 ex |
