An anti-evaporation tube for use with an in vitro diagnostics automation system is provided. The anti-evaporation tube includes an anti-evaporation tube body configured to be automatically inserted in a container and to limit evaporation of one or more fluids in the container and an alignment portion disposed on the anti-evaporation tube body. The alignment portion is configured to automatically align the anti-evaporation tube body within the container and cause the anti-evaporation tube body to remain aligned within the container. The alignment portion includes one or more openings disposed on an upper area of the anti-evaporation tube body. The one or more openings are configured to provide ventilation.
