Method and device for the 3D reconstruction of the distribution of fluorescent elements

摘要

The method involves detecting a fluorescence signal transmitted by a fluorophore (22) in its medium environment (20) for position torque of a femto-second type radiation source (8) e.g. pulsated laser diode, and a detector (4) e.g. camera. A variable value independent of life-time is calculated from the detected fluorescence signal. Position, spatial distribution and concentration of the fluorophore in the environment are determined from the variable value. An independent claim is also included for a device for processing a fluorescence signal.