IN VIVO INCORPORATION OF AN UNNATURAL AMINO ACID COMPRISING A 1,2-AMINOTHIOL GROUP
摘要
The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids that comprise a 1,2 aminothiol group into polypeptides. The invention provides translation systems in which polypeptides comprising unnatural amino acids that comprise a 1,2 aminothiol group can be produced. The invention also provides methods for producing polypeptides containing unnatural amino acids that comprise a 1,2 aminothiol group. Also provided by the invention are compositions comprising orthogonal aminoacyl-tRNA synthetases that preferentially aminoacylate a cognate orthogonal tRNA with unnatural amino acids that comprise a 1,2 aminothiol group. The invention provides methods for the synthesis of the unnatural amino acid 2-amino-3 -(4-(2-amino-3 -mercaptopropan-amido)phenyl)-propanoic acid.
申请公布号
WO2009151491(A9)
申请公布日期
2010.01.28
申请号
WO2009US01272
申请日期
2009.02.27
申请人
THE SCRIPPS RESEARCH INSTITUTE;FICHT, SIMON;JAHNZ, MICHAEL;GRUNEWALD, JAN;SCHILLER, STEFAN;SCHULTZ, PETER, G.
发明人
FICHT, SIMON;JAHNZ, MICHAEL;GRUNEWALD, JAN;SCHILLER, STEFAN;SCHULTZ, PETER, G.