发明名称 |
Kits and methods for generating 5' capped RNA |
摘要 |
<p>The present invention relates to kits and methods for efficiently generating 5′capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production of 5′-capped RNA having a modified cap nucleotide and for in vitro or in vivo production of polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate, and methods and kits for using a capping enzyme system and modified cap nucleotides for labeling uncapped RNA comprising primary RNA transcripts or RNA having a 5′-diphosphate with detectable dye or enzyme moieties.</p> |
申请公布号 |
EP2010659(A2) |
申请公布日期 |
2009.01.07 |
申请号 |
EP20070775441 |
申请日期 |
2007.04.16 |
申请人 |
EPICENTRE TECHNOLOGIES |
发明人 |
JENDRISAK, JEROME;MEIS, RONALD;DAHL, GARY |
分类号 |
C12N15/11;C12N15/113;C12P19/34 |
主分类号 |
C12N15/11 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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