Self-Reproducing Hybrid Plants

摘要

Compositions and methods for the production of self-reproducing hybrid plants are provided. Compositions include suppression cassettes encoding polynucleotides and promoters that result in the MiMe diploid gamete phenotype compositions and suppression cassettes and expression cassettes useful for genome elimination of a parental diploid gamete in a fertilized zygote. The methods involve crossing a first plant comprising a first suppression cassette responsible for producing the MiMe diploid gamete phenotype and a first expression cassette expressing an active CENH3 mutant with a second plant comprising a second suppression cassette that reduces the level of wild-type CENH3 and a second expression cassette comprising a polynucleotide expressing CENH3 specifically in the ovule. Self fertilization of the resultant progeny plant results in the elimination of the female diploid genome in the zygote and normal development of the endosperm. Additionally provided are plants and seeds produced by the methods of the invention.

主权项

1. A method for producing a self-reproducing hybrid plant comprising:
a) obtaining a first plant comprising in its genome a first suppression cassette and a first expression cassette,
i) wherein said first suppression cassette comprises at least one first silencing element wherein said first silencing element, when expressed by said self-reproducing hybrid plant, reduces the level of at least one target sequence, wherein said target sequence comprises a member selected from the group consisting of,
A) a gene critical to meiotic second division reduction,B) a gene critical to meiotic recombination, andC) a gene critical to meiotic chromosome segregation,ii) wherein said first expression cassette comprises a nucleic acid molecule encoding an active centromere-specific mutant polypeptide, wherein said active centromere-specific mutant polypeptide is expressed in said self-reproducing hybrid plant; b) obtaining a second plant comprising in its genome a second suppression cassette and a second expression cassette,
i) wherein said second suppression cassette comprises at least one second silencing element, wherein said second silencing element, when expressed by said self-reproducing hybrid plant, reduces the level of a wild-type centromere-specific polypeptide or a homolog thereof;ii) wherein said second expression cassette comprises a nucleic acid molecule encoding a wild-type centromere-specific polypeptide or homolog thereof, wherein said centromere-specific polypeptide is expressed in said self-reproducing hybrid plant; and c) crossing said first plant with said second plant thereby producing said self-reproducing hybrid plant.