METHOD OF INCREASING PRODUCTIVITY OF EUCARYOTIC CELLS IN PRODUCTION OF RECOMBINANT FVIII

摘要

FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Disclosed is a method of increasing cell-specific productivity of recombinant factor VIII (rFVIII) produced in an eukaryotic cell suspension during culturing of said eucaryotic cell suspension in a culturing medium. Method involves culturing a cell suspension obtained by inducing mechanical shearing force on mammalian cell suspension culture by applying specific power of 3 W/m. Culturing medium contains not more than 500 mcM CaCl, non-ionic detergent.EFFECT: invention provides high output of protein produced by cultured cells.14 cl, 5 dwg, 2 tbl, 4 ex