COMPOSITIONS AND METHODS FOR INHIBITING GENE EXPRESSIONS

摘要

A combined packing and assembly method that efficiently packs ribonucleic acid (RNA) into virus like particles (VLPs) has been developed. The VLPs can spontaneously assemble and load RNA in vivo, efficiently packaging specifically designed RNAs at high densities and with high purity. In some embodiments the RNA is capable of interference activity, or is a precursor of a RNA capable of causing interference activity. Compositions and methods for the efficient expression, production and purification of VLP-RNAs are provided. VLP-RNAs can be used for the storage of RNA for long periods, and provide the ability to deliver RNA in stable form that is readily taken up by cells.

主权项

1. A virus like particle (VLP) containing ribonucleic acid (RNA) comprising
a) a virus like particle formed from coat proteins of a bacteriophage; and b) RNA comprising a capsid binding tag; and wherein the coat proteins of the bacteriophage self-assemble to encapsidate three of the RNAs per VLP.