| 发明名称 |
OLIGONUCLEOTIDE-BASED PROBES AND METHODS FOR DETECTION OF MICROBES |
| 摘要 |
The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., endonuclease) substrates, and probes and compositions useful in detection assays. |
| 申请公布号 |
US2016340717(A1) |
申请公布日期 |
2016.11.24 |
| 申请号 |
US201515117414 |
申请日期 |
2015.02.09 |
| 申请人 |
UNIVERSITY OF IOWA RESEARCH |
发明人 |
McNamara James O.;Flenker Katie R.;Kim Hyeon;Horswill Alexander R.;Hernandez Frank J.;Behlke Mark;Huang Lingyan;Owczarzy Richard;Burghardt Elliot;Clark Karen |
| 分类号 |
C12Q1/68;G01N21/64;C12Q1/70;A61K49/00;G01N33/58 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A probe for detecting a bacterial or viral endonuclease comprising an oligonucleotide of 2-30 nucleotides in length, at least one fluorophore operably linked to the oligonucleotide, and at least one fluorescence quencher operably linked to the oligonucleotide, wherein the oligonucleotide is capable of being specifically cleaved by the bacterial or viral endonuclease but not by a mammalian nuclease or a non-bacterial or non-viral nuclease. |
| 地址 |
Iowa City IA US |
