Methods and compositions are presented for the generation of targeted alterations in a plant genome using double-stranded homogeneous oligonucleotides containing a single type of nucleotide. These methods can be used to correct mutations, introduce mutations and/or alter gene activity in a plant cell. A cell-free assay system for monitoring genetic alteration using the oligonucleotides of the invention is also presented.
申请公布号
WO0187914(A2)
申请公布日期
2001.11.22
申请号
WO2001US16152
申请日期
2001.05.17
申请人
UNIVERSITY OF DELAWARE;MAY, GREGORY, D.;KMIEC, ERIC, B.;RICE, MICHAEL, C.
发明人
MAY, GREGORY, D.;KMIEC, ERIC, B.;RICE, MICHAEL, C.