Method for producing vanillic acid and vanillin from waste residue of rice bran oil by fermentation and biotransformation

摘要

The invention provides a method for the producing vanillic acid and vanillin comprising:
(1) culturing Aspergillus niger CGMCC 0774 in a fermentor;
(2) adding waste residue of rice bran oil containing ferulic acid to the fermentation broth of step (1) to obtain a converted liquid containing vanillic acid;
(3) filtrating the converted liquid of step (2) with a membrane, and autoclaving after supplementation with glucose;
(4) culturing Pycnoporus cinnabarinus CGMCC 1115 or a mutant thereof in a fermentor to obtain wet mycelium;
(5) mixing the wet mycelium of step (4) with the converted liquid of step (3) to perform a conversion reaction to produce a solution of vanillin; and
(6) extracting vanillin from the solution of vanillin of step (5).