DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCE USING COUPLED LIGASE DETECTION AND POLYMERASE CHAIN REACTION

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting the difference of the detected sequences. SOLUTION: This invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction (LDR) and polymerase chain reaction (PCR). One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a ligase detection reaction coupled to a secondary polymerase chain reaction coupled to primary polymerase chain reaction. A third aspect of the present invention involves a secondary polymerase chain reaction coupled to a primary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences. COPYRIGHT: (C)2008,JPO&INPIT