| 摘要 |
A system and method directed to DNA amplification with optional in situ purification, sequencing and/or detection, or a system compatible with integrated, post-amplification purification and or sequencing by capillary electrophoresis and other methods. The device is a single, helical channel formed of fused silica with heat zones defined about fixed arcs of the helix inner and/or outer circumference. The length of the helical channel and the cycle number and dwell time may be varied by altering the pitch of the helix within the cylindrical substrate. In another embodiment, the heat zone arcs lengths are also variable. In still another embodiment, multiple helical channels are available in parallel within the same structure. Separation channels may be integrated on the device for post-amplification purification and/or sequencing. One or more detection schemes may be provided on the device or seamlessly integrated with the device, for monitoring amplification and/or detecting specific products. |
| 主权项 |
1. A method of conducting a Polymerase Chain Reaction (PCR) amplification, the method comprising:
providing a Nucleic Acid Amplification Device (NAAD), the NAAD comprising a cartridge that includes a helical channel monolith which is a fused silica rod or tube having a helical capillary channel extending within the fused silica rod or tube from an inlet end to an outlet end; and three heaters, each adjacent to the fused silica rod or tube, extending a length of the fused silica rod or tube from the inlet end to the outlet end, and extending in arcs about the fused silica rod or tube, wherein the three heaters divide the fused silica rod or tube into three different thermal zones that, individually, extend from the inlet end to the outlet end of the fused silica rod or tube; differentially heating the three thermal zones of the NAAD thereby providing a denaturation zone, an annealing zone, and an elongation zone; introducing a mixture of a nucleic acid sample and PCR reagents to the helical capillary channel; then repeatedly cycling the mixture of the nucleic acid sample and PCR reagents through the denaturation zone, the annealing zone, and the elongation zone, wherein each cycle has the same dwell time; and then collecting a product of the PCR amplification. |
