| 摘要 |
The present invention is drawn to methods of preparing crosslinked collagen microspheres and microparticles, where a solution of soluble collagen in an aqueous acidic medium is combined with an emulsifying agent to form a first mixture of the soluble collagen solution and the emulsifying agent, the first mixture then is combined with a hydrophobic liquid under conditions effective to form water-in-oil emulsion of the hydrophobic liquid and the first mixture, the water-in-oil emulsion then is added to a buffered alcohol solution under conditions effective to form a dispersion of a continuous liquid phase and a dispersed solid phase of collagen fibers reconstituted from the soluble collagen solution, the liquid phase then is removed from the dispersion to provide collagen microspheres, the collagen microspheres then are crosslinked to form crosslinked collagen microspheres, and the crosslinked collagen microspheres then are optionally dried to form crosslinked collagen microparticles.
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