发明名称 |
METHOD FOR MASS PROPAGATION OF OPLOPANAX ELATUS NAKAI USING TISSUE-DERIVED SOMATIC EMBRYO |
摘要 |
A method for mass-producing an Oplopanax elatus Nakai plantlet is provided to mass-propagate the Oplopanax elatus Nakai effectively from embryogenetic callus derived from an Oplopanax elatus Nakai tissue, thereby preserving endangered Oplopanax elatus Nakai. A method for mass-producing an Oplopanax elatus Nakai plantlet comprises the steps of: (a) explanting a tissue segment of Oplopanax elatus Nakai a cell tissue cultures in an MS culture medium where a plant growth control material including TDZ is added to induce embryogenetic callus; (b) culturing the induced embryogenetic callus in a liquid culture medium or a solid culture medium to induce somatic embryos; (c) culturing the induced somatic embryos in a liquid culture medium or a solid culture medium to propagate them; (d) culturing the propagated somatic embryos in a liquid culture medium or a solid culture medium having a pH of 5.0-5.75 to redifferentiate a plantlet; and (e) acclimatizing the redifferentiated plantlet in a soil where bed soil and sand are mixed in a ratio of 1:1, wherein the culture medium of the step(a) is an MS culture medium where 2,4-D or NAA is further added as the plant growth controlling material. Further, the MS culture medium comprises 10-30 mg/l of sucrose as carbon source.
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申请公布号 |
KR100815439(B1) |
申请公布日期 |
2008.03.20 |
申请号 |
KR20070014897 |
申请日期 |
2007.02.13 |
申请人 |
KNU-INDUSTRY COOPERATION FOUNDATION |
发明人 |
YU, CHANG YEON;KIM, MYOUNG JO;PARK, JAE GUN;CHO, DONG HA;LIM, JUNG DAE;KIM, HEE YOUNG |
分类号 |
A01H4/00;A01G1/00 |
主分类号 |
A01H4/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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