METHODS OF ASSESSING AND IDENTIFYING OR EVOLVING CONDITIONALLY ACTIVE THERAPEUTIC PROTEINS

摘要

PROBLEM TO BE SOLVED: To provide methods of evolving or selecting therapeutic proteins that exhibit reduced adverse side-effects, and resulting proteins.SOLUTION: Provided herein is an in vitro assay for identifying conditionally active therapeutic proteins that exhibit better activity within one in vivo environment compared to another in vivo environment. A method includes steps of; a) testing activity of a protein under conditions in which normal or increased activity is desired; b) testing activity of the protein under conditions in which reduced activity compared to normal is desired; and c) comparing the activity in a) with b) and selecting/identifying a protein that has greater activity in a) compared to b). The selected/identified protein is a conditionally active protein.SELECTED DRAWING: None