Self-Referencing Sensor for Chemical Detection

摘要

Provided herein is a sensing apparatus comprising, at least one LSPR light source, at least one detector, and at least one sensor for LSPR detection of a target chemical. The sensor comprises a substantially transparent, porous membrane having nanoparticles immobilized on the surface of its pores, the nanoparticles being functionalized with one or more capture molecules. There is further provided a self-referencing sensor for distinguishing non-specific signals from analyte binding signals. The self-referencing sensor comprising one or more nanoparticles having at least two distinct LSPR signals.

主权项

1. A self-referencing sensor for detection of an analyte binding signal and simultaneous detection of non-specific signals, comprising:
one or more types of nanoparticles, the one or more types of nanoparticles having at least two distinct LSPR signals, wherein at least one nanoparticle type of the one or more types of nanoparticles includes a capture molecule to bind to an analyte and whereby the optical signal generated by an analyte bound to the at least one nanoparticle type can be distinguished from non-specific signals, by a difference in a resulting optical spectrum change between the two distinct LSPR signals.