CONSTRUCTS FOR EXPRESSING BIOLOGICAL MOLECULES THAT INTEGRATE INTO BACTERIAL MICROCOMPARTMENTS

摘要

A conserved region of sequence in bacterial microcompartment (BMC) enzymes and proteins was identified. Peptide sequences derived from this conserved region of native BMC proteins and enzymes appear to target the hexameric facets of BMC shell proteins. These peptides were predicted to share general properties of a predicted alpha helical conformation, flanked by poorly conserved segment(s) of primary structure); for each type of encapsulated protein, and for each functionally distinct BMC. These peptides can be used as targeting signals for integrating biomolecules and molecules into bacterial microcompartments or for attaching molecules or biomolecules to native or non-native bacterial microcompartment shell proteins.

主权项

1. A polynucleotide encoding a heterologous fusion protein targeted to a bacterial microcompartment, comprising:
a first nucleotide sequence that encodes a bacterial microcompartment targeting peptide; a second nucleotide sequence that encodes a heterologous protein to be targeted to a bacterial microcompartment; and a promoter sequence operably linked to the first nucleotide sequence and the second nucleotide sequence.