发明名称 |
KIT FOR PRODUCING TRANSPARENTIZED BIOLOGICAL SPECIMEN, AND METHOD FOR PRODUCING TRANSPARENTIZED BIOLOGICAL SPECIMEN |
摘要 |
Provided are: a method and a kit for producing a biological specimen clear, which enable the clearing within a short time and by which a wide variety of biological specimens can be cleared; and a method and a kit for preserving a cleared biological specimen. A method for producing a cleared biological specimen, comprising steps as mentioned in items (A) to (C). (A) Step (1) of treating a material to be cleared with a fixing solution that is an aqueous solution containing paraformaldehyde or the like, a nonionic surfactant (at a concentration of 1% or more) and an alkali and optionally containing a buffering agent to produce the material that is cleared is involved. (B) Step (2) of treating the material that is produced in step (1) or a material to be cleared which is produced by a conventional fixing method with a clearing accelerator solution that is an aqueous solution containing a nonionic surfactant (at a concentration of 1% or more) and an alkali to produce the material that is cleared is involved. (C) Step (3) of treating the material produced in step (1) or (2) with a preservation solution that is an aqueous solution containing a nonionic surfactant (at a concentration of 1% or more) and a polyhydric alcohol to produce the material that is cleared is involved. A kit which can be used in the method. |
申请公布号 |
EP2926658(A4) |
申请公布日期 |
2016.08.31 |
申请号 |
EP20130850135 |
申请日期 |
2013.10.30 |
申请人 |
KANAZAWA MEDICAL UNIVERSITY |
发明人 |
HATTA, TOSHIHISA;UCHISHIBA, MAIMI;HIGASHI, NOBUAKI;SHIMADA, HIROKI;SHIMAMURA, ERIKO |
分类号 |
A01N1/02;G01N1/30 |
主分类号 |
A01N1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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