Methods of fluorescence imaging, computer program, computerprogram product and fluorescence imaging system

摘要

Method of fluorescence imaging comprising: a) illuminating a sample to excite its fluorescence and acquiring an image thereof; b) based on spectral and spatial information from the sample's fluorescence image, segmenting the image into regions of similar spectral properties; c) for each image segment, exciting the fluorescence of the corresponding sample region, and detecting the corresponding fluorescence; d) based on modelling, determining expected fluorescence parameters from the fluorescence signals detected for each region; e) scanning the sample and determining final fluorescence parameters based on said expected fluorescence parameters from step d).