PRODUCTION OF OLIGOSACCHARIDES

摘要

The present invention relates to the use of one or more glycosidases in the process for the production and/or purification of a produced desired oligosaccharide. The process is preferably a microbial fermentation process using a host microorganism, which may also comprise nucleic acids expressing sugar catabolic pathway proteins suitable for the degradation of saccharides otherwise hindering the purification of the desired oligosaccharide.

主权项

1. A process for producing an oligosaccharide using a host microorganism, wherein said oligosaccharide is not naturally occurring in said host cell, the process comprising the steps of:
a) cultivating a host microorganism suitable for the production of a desired oligosaccharide under conditions and in a medium permissive for the production of said oligosaccharide, whereby the oligosaccharide and, where applicable, biosynthetic saccharide intermediates and/or side products are produced; b) using a glycosidase in the medium the host microorganism is cultivated in, to degrade biosynthetic saccharide intermediates and/or saccharide side products and/or unused saccharide substrates, and c) recovering the desired oligosaccharide.