发明名称 |
NEW METHOD FOR INDUCING DOPAMINE-PRODUCING NEURAL PRECURSOR CELLS |
摘要 |
The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGF² inhibitor, SHH signal-stimulating agent, FGF8, and GSK3² inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor. |
申请公布号 |
EP3042951(A1) |
申请公布日期 |
2016.07.13 |
申请号 |
EP20140841854 |
申请日期 |
2014.09.04 |
申请人 |
KYOTO UNIVERSITY;OSAKA UNIVERSITY;EISAI R&D MANAGEMENT CO., LTD. |
发明人 |
TAKAHASHI, JUN;DOI, DAISUKE;SAMATA, BUMPEI;SEKIGUCHI, KIYOTOSHI;ONO, YUICHI |
分类号 |
C12N5/0797;A61K35/30;A61P25/16 |
主分类号 |
C12N5/0797 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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