Method for direct detection of lipid binding agents in membrane

摘要

The method for direct detection of lipid binding agents in membrane includes dissolving a lipid in an organic solvent in a container; evaporating off the organic solvent to create a lipid film on the inside wall of the container; adding a buffer solution; adding a known volume of a membrane active agent to the buffer solution; incubating the solution in the dark for a period of time; removing the buffer solution from the container to provide a solution of sample A; washing the container with a buffer solution; adding an organic solvent while stirring the container to dissolve the lipid film to create a homogenous solution B; and measuring the absorbance of the samples A and B by absorption spectroscopy. The method may further include developing a universal probability function to test and quantify a membrane-based cytotoxicity of general drug candidates.

主权项

1. A method for direct detection of lipid binding agents in membrane, comprising the steps of:
dissolving a lipid in an organic solvent in a container; evaporating off the organic solvent to create a lipid film on the inside wall of the container; adding a buffer solution; adding a known volume of a membrane active agent to the buffer solution; incubating the solution in the dark for a period of time; removing the buffer solution from the container to provide a solution of sample A; washing the container with a buffer solution; adding an organic solvent while stirring the container to dissolve the lipid film to create a homogenous solution B; measuring the absorbance of the samples A and homogenous solution B by absorption spectroscopy; and quantifying the lipid binding energies by the membrane active agents from the absorbance measurements, wherein the membrane active agent is selected from the group consisting of colchicine, aptamer 5′-AAAAGA-3′ and 5′-AAAGAC-3.