| 摘要 |
The chamber (10) for the biological component (100) of a bio-artificial liver (200) is configured to allow proliferation of the biological component in situ, cryopreservation of the biological component in situ and perfusion of the biological component in situ. The cahmber may comprise one or more heat exchange elements 38 and a helically wound fluid transport system 30 comprising a tube which is semi-permeable to gases. The biological component may comprise a matrix of Hep G2 cell line cells and one or more density modifiers such as alginate or glass beads. Also disclosed is a method of manufacturing a biological component using the matrix, a method of proliferating cells seeding in the matrix and a method of cryopreserving proliferated cells in the chamber. |
