| 摘要 |
The present invention relates to a method for detecting an analyte in a sample, comprising (a) contacting said sample with at least one set of at least first, second and third proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte, the nucleic acid domain of said third proximity probe being a splint which is capable of hybridising at least to the nucleic acid domains of said first and second proximity probes, wherein when all of the at least three proximity probes bind to said analyte, the nucleic acid domains of said first and second proximity probes are conjugatable by means of an interaction mediated by said hybridised splint of said third proximity probe; (b) conjugating the nucleic acids, of said first and second proximity probes; and (c) detecting said conjugation. Also provided is a kit for use in such a method.
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