METHOD FOR PRODUCING PROTEIN BY PRECIPITATION

摘要

The present invention provides a method for producing a target protein in the form of a fusion protein at a high recovery ratio. The present invention relates to a method for producing a fusion protein made of a protein having a self-assembly capability and a target protein comprising the following steps (1) to (4):
(1) preparing a solution containing the fusion protein;
(2) adjusting a pH of the solution obtained in step (1) to such a pH that a recovery ratio calculated according to the following equation is 10% or more, where the recovery ratio % = an amount of the fusion protein in a solution obtained is step 4 / the amount of the fusion protein in the solution obtained in step 4 + an amount of the fusion protein in a solution after solid separation in step 3 × 100 ;
(3) separating a solid from the solution obtained in step (2); and
(4) dissolving the solid separated in step (3) into a solution having a pH of 12 or below but higher than the pH of the solution obtained in step (2) by 0.1 or more.