发明名称 |
METHOD FOR CONSTRUCTING LONG FRAGMENT DNA LIBRARY |
摘要 |
Disclosed is a method for constructing a long fragment DNA library, comprising the following steps: 1) breaking a long fragment DNA into target fragments of 3-10 kb by transposase, then amplifying the target fragments, and obtaining target fragment amplification products containing dUTP; 2) amplifying the dUTP in the products by removing the target fragments, fragmenting the target fragments secondarily into DNA short fragments of 300-1200 bp; 3) connecting both ends of the DNA short fragments with sequencing linker single chains A and sequencing linker single chains B respectively; and obtaining connecting sequencing linker products; and 4) PCR amplifying the connecting sequencing linker products, to obtain amplification products. |
申请公布号 |
WO2016169431(A1) |
申请公布日期 |
2016.10.27 |
申请号 |
WO2016CN79278 |
申请日期 |
2016.04.14 |
申请人 |
BGI SHENZHEN;BGI SHENZHEN CO., LIMITED |
发明人 |
WANG, Ou;CHANG, Cankun;LIN, Lin;JIANG, Hui;ZHANG, Wenwei |
分类号 |
C40B50/06;C12N15/10;C40B40/06 |
主分类号 |
C40B50/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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