发明名称 |
Characterising polypeptides |
摘要 |
Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of: (a) contacting a sample comprising one or more polypeptides with a lysine reactive agent to cap epsilon-amino groups; (b) optionally reacting the sample of polypeptides with an amine reactive reagent to block alpha-amino groups; (c) digesting the sample of polypeptides with a cleavage reagent to produce peptide fragments; (d) optionally deactivating the cleavage reagent; (e) removing those peptides having uncapped or unblocked amino groups; and (f) recovering the N-terminal peptides.
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申请公布号 |
US2005042676(A1) |
申请公布日期 |
2005.02.24 |
申请号 |
US20040479855 |
申请日期 |
2004.10.18 |
申请人 |
HAMON CHRISTIAN;THOMPSON ANDREW;KUHN KARSTEN;NEUMANN THOMAS;JOUBERT RICHARD;JOHNSTONE ROBERT;SCHMIDT GUNIER |
发明人 |
HAMON CHRISTIAN;THOMPSON ANDREW;KUHN KARSTEN;NEUMANN THOMAS;JOUBERT RICHARD;JOHNSTONE ROBERT;SCHMIDT GUNIER |
分类号 |
C07D213/71;C07D215/36;C07D239/38;C07D241/18;C07D251/58;C07K1/107;C07K1/12;C12Q1/02;C12Q1/37;G01N33/68;(IPC1-7):G01N33/53 |
主分类号 |
C07D213/71 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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