| 摘要 |
The invention relates to methods for increasing the titer of a protein of interest in a cell as well as enhancing production and purification of optimized biomolecules, the component of which is, inter alia, the CH3 domain. In biomolecules, the C-terminal amino acid(s), e.g. the C-terminal lysine, frequently separate(s). The mostly incomplete processing of, for example, the heavy chain of antibodies results in products being heterogeneous. In order to avoid said product heterogeneity, the respective codon of the C-terminal lysine of the heavy antibody chain is deleted using recombinant DNA technology. Said optimized antibodies result in an increased product titer than in the wild type. In addition, said optimized antibodies are advantageous in the purification process as a result of an improved elution behavior due to the reduced charge heterogeneity. |
