| 摘要 |
The purpose of the present invention is, in the analysis of a nucleic acid of interest employing a nucleic acid amplification reaction, to reduce the sample-to-sample variations of the amount of amplified nucleic acids to a value falling within the detection dynamic range of an analysis device in a simple manner. A specified amount of magnetic beads 115, which serve as carriers, are brought into contact with an amplification solution after the completion of the reaction. The magnetic beads 115 can bind specifically to both of amplified nucleic acid molecules and unreacted substrate molecules both of which are produced in a step of amplifying the nucleic acid of interest by a nucleic acid amplification reaction using a substrate as a raw material molecule, and can bind predominantly to the unreacted substrate molecules over the amplified nucleic acid molecules. A binding reaction is carried out to bind the amplified nucleic acid molecules and/or the unreacted substrate molecules to the surfaces of the magnetic beads 115. After the completion of the binding reaction, the magnetic beads 115 are separated from a binding reaction solution with a magnet 116, and the resultant supernatant 117 is analyzed. |
