DIAGNOSTIC METHODS FOR INFECTIOUS DISEASE USING ENDOGENOUS GENE EXPRESSION

摘要

Disclosed are methods of diagnosis of a pathogen-associated disease. These methods comprise: providing a biological sample from a human subject; determining presence, absence and/or quantity of a bacterial pathogen, a viral pathogen, or a combination thereof, by a pathogen culture, a serum antibody detection test, a pathogen antigen detection test, a pathogen DNA and/or RNA detection test, or a combination thereof; determining in the sample, expression levels of at least one endogenous gene in which aberrant expression levels are associated with infection with a pathogen, by a microarray hybridization assay, RNA-seq assay, polymerase chain reaction assay, a LAMP assay, a ligase chain reaction assay, a Southern, Northern, or Western blot assay, an ELISA or a combination thereof. The subject can be diagnosed with the disease if the subject comprises the pathogen and an aberrant level of expression of an endogenous gene.

主权项

1. A method of deterinining etiology of a pathogen-associated disease in a human subject, comprising:
a) providing at least one biological sample from a human subject; b) detecting in the sample the presence, absence and/or quantity of a bacterial pathogen, a viral pathogen, or a combination thereof, by a pathogen culture, a serum antibody detection test, a pathogen antigen detection test, a pathogen DNA detection test, a pathogen RNA detection test, or a combination thereof; c) detecting in the sample expression levels of at least one endogenous gene in which aberrant expression levels are associated with infection with a bacterial pathogen, a viral pathogen or a combination thereof by a microarray hybridization assay, an RNA-seq assay, a polymerase chain reaction assay, a LAMP assay, a ligase chain reaction assay, a Southern blot assay, a Northern blot assay, a Western blot assay, an enzyme-linked immunosorbent assay (ELISA) or as combination thereof, wherein the etiology of the pathogen-associated disease is determined to be bacterial if the sample a) comprises a bacterial pathogen and b) exhibits an aberrant level of expression of at least 1.5 fold increase or decrease of at least one endogenous gene associated with bacterial infection, and wherein the etiology of the pathogen-associated disease is determined to be viral if the sample a) comprises a viral pathogen and b) exhibits an aberrant level of expression of at least 1.5 fold increase or decrease of at least one endogenous gene associated with viral infection.