Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing

摘要

Disclosed are methods for shearing and tagging chromatin DNA. The disclosed methods include contacting chromatin DNA with at least one transposome, that includes a transposase enzyme. The transposon is made up of a first DNA molecule that includes a first transposase recognition site and a second DNA molecule that includes a second transposase recognition site, wherein the transposase integrates the first and second DNA molecules into chromatin DNA. The first and second DNA molecules of the transposon can be disconnected, such that upon integration of the transposon the chromatin bound DNA is sheared and tagged with the first and second DNA molecules, for example to prepare a library of sheared and tagged chromatin DNA fragments.

主权项

1. A method for shearing and tagging chromatin DNA,
comprising: contacting chromatin DNA, under conditions that permit integration of a transposon into chromatin DNA, with at least one transposome, the transposome comprising: at least one transposase; and a transposon comprising:
a first DNA molecule comprising a first transposase recognition site; anda second DNA molecule comprising a second transposase recognition site wherein the at least one transposase integrates the first and second DNA molecules into chromatin DNA,thereby shearing and tagging chromatin DNA with the first and second DNA molecules.