METHODS OF ALLELE TYPING

摘要

The present invention relates to methods for reducing the ambiguity in human leukocyte antigen (HLA) allele identification. In particular, the methods comprise using sequence specific oligonucleotide (SSO) techniques to determine a first set of possible HLA alleles. The methods further comprise using sequence-based typing (SBT) to obtain a second set of possible HLA alleles. The two sets of the possible HLA alleles are then combined to determine at least one common allele identified in the both the SSO and SBT assays, thus reducing the ambiguity associated with current HLA typing procedures.