METHODS AND COMPOSITIONS FOR WHOLE TRANSCRIPTOME AMPLIFICATION

摘要

The disclosure provides for methods, compositions, systems, devices, and kits for whole transcriptome amplification using stochastic barcodes.

主权项

1. A method for labeling a plurality of targets from a sample, comprising:
hybridizing the plurality of targets from the sample with a plurality of nucleic acids each comprising a first universal label; extending the plurality of nucleic acids to generate a plurality of first strand polynucleotides; synthesizing a plurality of second strand polynucleotides using the plurality of first strand polynucleotides as templates to generate a plurality of double-stranded polynucleotides; ligating an adaptor to the plurality of double-stranded polynucleotides, wherein said adaptor comprises a second universal label; and amplifying the plurality of double-stranded polynucleotides using the first universal label and the second universal label, thereby generating a plurality of amplicons comprising the plurality of targets.