LOW-FREQUENCY MUTATION ENRICHMENT SEQUENCING METHOD FOR FREE TARGET DNA IN PLASMA DNA

摘要

The present invention provides a low-frequency mutation enrichment sequencing method for free target DNA in plasma, comprising plasma DNA extraction and library construction, general library TT COLD PCR amplification enrichment, probe enrichment capture, PCR and computer sequencing of captured products, and positive and negative double-strand error-correction low-frequency information analysis.