PRODUCTION OF HUMAN PULMONARY SURFACTANT PROTEIN B IN PLANTS

摘要

The invention is directed to a method of producing pulmonary surfactant protein-B (SP-B) isoforms fragments and analogues thereof in a plant cell, and to expression cassettes for expression of the SP-B proteins in plants. The expression cassettes may include various elements for improved expression, stability of the expressed protein or efficient purification of the expressed protein, including signal sequences, protease cleavage sites for release of the target protein, trafficking peptides for trafficking of the expressed protein to various plant compartments, and/or various tags.

主权项

1. A method of producing a protein in a plant cell, wherein the protein is selected from the group consisting of pulmonary surfactant protein-B (SP-B) pre-protein or a functional fragment or analog thereof, and SP-B mature peptide or a functional fragment or analog thereof, the method comprising the steps of:
(i) providing a nucleic acid sequence comprising a polynucleotide sequence encoding the protein; (ii) introducing the nucleic acid sequence into an expression vector adapted to express a polypeptide in a plant cell; (iii) introducing the expression vector of step (ii) into a plant cell; (iv) expressing the protein in the plant cell of step (iii); and (v) harvesting the protein from the plant cell.