发明名称 |
PROTEIN EXPRESSION IN RODENT CELLS |
摘要 |
A method is described for the expression of heterologous polypeptides in rodent cells. The method comprises the oriP/EBNA-1 episomal replication and maintenance system of the Epstein-Barr-Virus (EBV). With the stable integration of an EBNA-1 -protein expression cassette under the control of a promoter into the genome of a rodent cell an EBNA-I -protein expression in the cells was obtained. The heterologous protein is expressed from an episome comprising an EBV origin of replication and a functional expression cassette of said heterologous protein. The invention further comprises transformed rodent cell lines, a method for the production of a heterologous protein in said cell lines and a kit for the construction of said cell lines.
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申请公布号 |
CA2624685(A1) |
申请公布日期 |
2007.05.03 |
申请号 |
CA20062624685 |
申请日期 |
2006.10.26 |
申请人 |
F. HOFFMANN-LA ROCHE AG |
发明人 |
KOPETZKI, ERHARD;GOEPFERT, ULRICH |
分类号 |
C12N15/869 |
主分类号 |
C12N15/869 |
代理机构 |
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