| 摘要 |
Non-saturated or non-saturated and orientated binding surfaces are provided, and methods and compositions for making non-saturated or non-saturated and orientated binding surfaces. Methods of making binding surfaces for preparing a support surface for an affinity assay are provided, including using non-saturated or non-saturated and orientated binding surfaces on paramagnetic microparticles. The methods include methods for making ligand::support coupler-based complexes by a process optionally employing a low input ratio of ligand to support coupler, by dilution, and by methods employing a dispersion and/or coating step using a block copolymer. Specific examples employing biotin-BSA and biotin-ovalbumin binding surfaces are provided, as well as strepavidin-coated microparticles and microparticles coated with capture moieties such as, for example, biotinylated immunoglobulins or fragments thereof. Other examples couple a ligand to the solid surface. Use of the methods and compositions in connection with a wide variety of analytes and capture moieties is provided. Non-saturated or non-saturated and orientated binding surfaces for immunoassays are provided. Further provided are dispersed microparticles and methods for making them. |
