HIGHLY ACTIVE SILICA MAGNETIC NANOPARTICLES FOR PURIFYING BIOMATERIAL AND PREPARATION METHOD THEREOF

摘要

The present invention relates to a method for preparing highly active silica magnetic nanoparticles, highly active silica magnetic nanoparticles prepared by the method, and a method of isolating nucleic acid using the highly active silica magnetic nanoparticles. The highly active silica magnetic nanoparticles prepared according to the present invention contain magnetic nanoparticles completely coated with silica, can be used as a reagent for isolating biomaterials, particularly, nucleic acids, and can isolate and purify nucleic acid in a high yield.

主权项

1. A method for preparing highly active silica magnetic nanoparticles comprising:
(a) ultrasonically dispersing a solution obtained by adding magnetic nanoparticles, an acid-base catalyst and a water-soluble hydrophobic solvent to a solvent mixture of distilled water and an alcohol; and (b) adding silane to the dispersed solution to prepare silica magnetic nanoparticles.