A METHOD OF DETECTING AND SEPARATING SERUM GAMMA-GLUTAMYL TRANSFERASE (GGT) ISOFORMS IN A SAMPLE OF BIOLOGICAL FLUID AND THE ENZYME ISOFORMS THEREBY OBTAINED

摘要

A method is described for detecting the gamma-glutamyl transferase enzyme isoforms (GGT, EC 2.3.2.2) in a sample of biological fluid, such as for example plasma or serum. The method comprises an HPLC separation step of the sample proteins based on the molecular size and a second step for detecting the GGT isoforms by post-column reaction with a GGT enzyme substrate capable of generating a detectable final product, preferably by spectrophotometric or fluorimetric means. The GGT isoforms can be separated by ultra-centrifugation, thereby obtaining three enzymatic isoforms characterised by molecular weights of approximately 2000, 940, and 140 KDa, respectively.