METHOD FOR IDENTIFYING AGENTS WHICH MODULATE GTPASE ACTIVITY INVOLVED IN INSULIN-STIMULATED GLUT4 TRANSLOCATION

摘要

The present invention is a method for identifying agents that modulate the GTPase activity of AS160. In the instant assay, AS160 or the GAP domain thereof is contacted with a test agent, in the presence of GTP-bound Rab (2A, 8A, 8B, 10, or 14), and the AS160 GAP domain-mediated hydrolysis of GTP to GDP is monitored.