发明名称 |
METHOD FOR MASS PRODUCTION OF FACTOR VII/VIIA |
摘要 |
A method for the mass production of human coagulation Factor VII. The method includes a) providing an expression vector carrying i) a dihydrofolate reductase promoter devoid of one or more CCGCC repeat sequences from the GC-rich region thereof and a dihydrofolate reductase (DHFR) gene operably linked thereto and ii) a cytomegalovirus (CMV) promoter and a human coagulation Factor VII gene operably linked thereto; b) obtaining a transformed host cell line containing the expression vector; and c) culturing the transfected host cell in the presence of a dihydrofolate reductase inhibitor to select cells which express human coagulation Factor VII with high efficiency; and d) adding sodium butyrate to the selected host cells. |
申请公布号 |
EP2633058(B1) |
申请公布日期 |
2016.07.20 |
申请号 |
EP20110836619 |
申请日期 |
2011.10.26 |
申请人 |
HANMI SCIENCE CO., LTD. |
发明人 |
KIM, CHANG HWAN;HONG, SUNG KAP;LEE, BYUNG SUN;HONG, SUNG HWAN;KWON, SE CHANG |
分类号 |
C12N15/85;C07K14/745;C12N15/12 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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