NOVEL VECTOR AND UTILIZATION OF THE SAME

摘要

The present invention provides a tool for reducing the time and labor required for obtaining a protein by recombinant expression of a gene in a host. A vector characterized by having two restriction recognition sequences different in nucleotide sequence both of which are recognized by a first restriction enzyme, one of the restriction recognition sequences comprising a part of a restriction recognition sequence recognized by a second restriction enzyme that is different from the first restriction enzyme, and the other one of the restriction recognition sequences not comprising the part of the restriction recognition sequence recognized by the second restriction enzyme. A method of producing a protein and/or a protein domain, using the above-described vector.