METHOD FOR IN VITRO CONSTRUCTING SALIVARY GLAND ORGANOIDS AND ACINUS-LIKE

摘要

Disclosed is a method for in vitro constructing biological engineered salivary gland organoids and acinus-like based on human minor salivary gland adult stem cells. It particularly refers to a method for obtaining organoids with salivary gland structures and functions by mixed cultivation of mesenchymal stem cells and epithelial stem/progenitor cells of the human minor salivary glands, or a method for obtaining acinus-like tissues with salivary gland acinus structures and functions by individual cultivation of epithelial stem/progenitor cells of the human minor salivary glands. It has the advantages that sampling sources are wide, it is easy to cut, and the trauma is small; a donor site in a mouth is concealed; materials are drawn from adult tissues; and autoplastic transplantation can be carried out through amplification in vitro. The method has a wide application prospect in the growth and development research of in vitro or in vivo salivary glands, research on a salivary gland disease model, research on physiological functions and pathological changes of the saliva and treatment of salivary gland diseases.